pH-Spectrum of
Enzyme-inhibitor-complex

Experimental:

System IS - (0.7 g/L Soybean inhibitor (1 mg inhibit 1.7 mg Trypsin 10000 BAEE) / dist. water),
System (IS+T) - (0.7 g/L Soybean inhibitor (1 mg inhibit 1.7 mg Trypsin 10000 BAEE) + 1.2 g/L Trypsin / dist. water),
System IC - (1.2 g/L Chicken Egg White inhibitor (1 mg inhibit 1 mg Trypsin 10000 BAEE) / dist. water),
System (IC+T) - (1.2 g/L Chicken Egg White inhibitor (1 mg inhibit 1 mg Trypsin) + 1.2 g/L Trypsin / dist. water)

Results:

Fingerprints

System IS: At a pH-value below 4 and above 12 the decomposition of the Soybean inhibitor begins.
System IC: At a pH-value below 3 and above 11 the decomposition of the Chicken Egg White inhibitor begins.

Systemprint

System (IS+T): The enzyme-inhibitor-complex forms in the pH area of 3.5 to 11.8.
System (IC+T): The enzyme-inhibitor-complex forms in the pH area of 4.3 to 11.8.